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quantikine mouse serum amyloid  (R&D Systems)


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    R&D Systems quantikine mouse serum amyloid
    Quantikine Mouse Serum Amyloid, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantikine+mouse+serum+amyloid/Mouse+Serum+Amyloid+A+Quantikine+ELISA+Kit/pmc13022470-131-25-31
    Average 94 stars, based on 41 article reviews
    quantikine mouse serum amyloid - by Bioz Stars, 2026-10
    94/100 stars

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    Article Snippet: Homogenates were centrifuged, and the resulting supernatant was collected and either analyzed immediately or stored at −80°C. serum amyloid A concentrations were measured using the Quantikine Mouse Serum Amyloid A Immunoassay (R&D Systems; MSAA00) according to the manufacturer's instructions with modifications for tissue samples; liver supernatants were diluted 1:10 in Calibrator Diluent RD5‐26.



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    Serum amyloid A expression and production is disrupted with loss of ALX/FPR2. ALX/FPR2 wild type (FPR2 +/+ ) or ALX/FPR2 knockout (FPR2 −/− ) male mice 8–12 weeks old were exposed to filtered air (FA) or 1 ppm ozone (O 3 ) for 3 h and necropsied 6 h after the start of the exposure. Lung tissue was collected for real‐time PCR of (A) <t>Saa</t> 1 , (B) Saa 2 , (C) Saa 3 , (D) Saa 4 . (E) Plasma was collected for <t>ELISA</t> analysis of total SAA. * p < 0.05, ** p < 0.01, *** p < 0.001, n = 4–7/group.
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    Serum amyloid A expression and production is disrupted with loss of ALX/FPR2. ALX/FPR2 wild type (FPR2 +/+ ) or ALX/FPR2 knockout (FPR2 −/− ) male mice 8–12 weeks old were exposed to filtered air (FA) or 1 ppm ozone (O 3 ) for 3 h and necropsied 6 h after the start of the exposure. Lung tissue was collected for real‐time PCR of (A) Saa 1 , (B) Saa 2 , (C) Saa 3 , (D) Saa 4 . (E) Plasma was collected for ELISA analysis of total SAA. * p < 0.05, ** p < 0.01, *** p < 0.001, n = 4–7/group.

    Journal: The FASEB Journal

    Article Title: ALX / FPR2 Contributes to Serum Amyloid A‐Induced Lung Neutrophil Recruitment Following Acute Ozone Exposure

    doi: 10.1096/fj.202402865R

    Figure Lengend Snippet: Serum amyloid A expression and production is disrupted with loss of ALX/FPR2. ALX/FPR2 wild type (FPR2 +/+ ) or ALX/FPR2 knockout (FPR2 −/− ) male mice 8–12 weeks old were exposed to filtered air (FA) or 1 ppm ozone (O 3 ) for 3 h and necropsied 6 h after the start of the exposure. Lung tissue was collected for real‐time PCR of (A) Saa 1 , (B) Saa 2 , (C) Saa 3 , (D) Saa 4 . (E) Plasma was collected for ELISA analysis of total SAA. * p < 0.05, ** p < 0.01, *** p < 0.001, n = 4–7/group.

    Article Snippet: SAA concentrations in plasma were analyzed by ELISA using a mouse SAA quantikine ELISA kit (R&D Systems, MN, USA) per the manufacturer's instructions.

    Techniques: Expressing, Knock-Out, Real-time Polymerase Chain Reaction, Clinical Proteomics, Enzyme-linked Immunosorbent Assay